ESR2 anticorps (AA 1-153)

N° du produit ABIN7464811

L’anticorps Souris Monoclonal anti-ESR2 a été validé pour WB, IHC, IF, FACS, ICC, IHC (p), IHC (fro), ChIP, IHC (fp) et DB. Il convient pour détecter ESR2 dans des échantillons de Humain.

Aperçu rapide pour ESR2 anticorps (AA 1-153) (ABIN7464811)

Antigène: ESR2 (Estrogen Receptor 2 (ESR2))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ESR2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Chromatin Immunoprecipitation (ChIP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Dot Blot (DB)

Classe de qualité: KO Validated

Clone: 14C8

Détail du produit anti-ESR2 anticorps

Épitope: AA 1-153

Specificité: Strong Erb staining in epithelial cell nuclei. Occasional weak to moderate staining is seen in surrounding stromal and endothelial cell nuclei. Sporadic light cytoplasmic staining is sometimes observed. We have also successfully detected ERb in colon and ovarian tumors using the same antibody.,Knockdown/Knockout validation was supported by references (PMID:27323858)

Réactivité croisée: Humain, Singe, Souris

Purification: Protein G purified

Immunogène: Amino acids 1-153 of human ER-beta expressed in E. coli.

Isotype: IgG2b

Information d'application

Indications d'application: For IHC, a recommended protocol is provided. For WB: Use at a concentration of 1-5 μg/mL. For ChIP: Use at an dependent assay. Optimal dilutions/concentrations should be determined by the researcher.

Commentaires:

Positive Control: Myc-DDK-tagged ESR2-transfected 293T

Validation: Comparison, KO/KD, Overexpression

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.23 mg/mL

Buffer: PBS, No Preservative

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur ESR2

Antigène: ESR2 (Estrogen Receptor 2 (ESR2))

Autre désignation: estrogen receptor 2

Sujet: Estrogen receptor 2 , ER-BETA , ESR-BETA , ESRB , ESTRB , Erb , NR3A2 , ODG8,This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq, Jul 2008]

Poids moléculaire: 59 kDa

ID gène: 2100

UniProt: Q92731

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling